diff --git a/Lib/http/cookies.py b/Lib/http/cookies.py
index 769541116993c4..660fec4f1be865 100644
--- a/Lib/http/cookies.py
+++ b/Lib/http/cookies.py
@@ -391,17 +391,21 @@ def __repr__(self):
         return '<%s: %s>' % (self.__class__.__name__, self.OutputString())
 
     def js_output(self, attrs=None):
+        import base64
         # Print javascript
         output_string = self.OutputString(attrs)
         if _has_control_character(output_string):
             raise CookieError("Control characters are not allowed in cookies")
+        # Base64-encode value to avoid template
+        # injection in cookie values.
+        output_encoded = base64.b64encode(output_string.encode('utf-8')).decode("ascii")
         return """
         <script type="text/javascript">
         <!-- begin hiding
-        document.cookie = \"%s\";
+        document.cookie = atob(\"%s\");
         // end hiding -->
         </script>
-        """ % (output_string.replace('"', r'\"'))
+        """ % (output_encoded,)
 
     def OutputString(self, attrs=None):
         # Build up our result
diff --git a/Lib/test/test_http_cookies.py b/Lib/test/test_http_cookies.py
index e2c7551c0b3341..cfcbc17bd6df80 100644
--- a/Lib/test/test_http_cookies.py
+++ b/Lib/test/test_http_cookies.py
@@ -1,5 +1,5 @@
 # Simple test suite for http/cookies.py
-
+import base64
 import copy
 import unittest
 import doctest
@@ -175,17 +175,19 @@ def test_load(self):
 
         self.assertEqual(C.output(['path']),
             'Set-Cookie: Customer="WILE_E_COYOTE"; Path=/acme')
-        self.assertEqual(C.js_output(), r"""
+        cookie_encoded = base64.b64encode(b'Customer="WILE_E_COYOTE"; Path=/acme; Version=1').decode('ascii')
+        self.assertEqual(C.js_output(), fr"""
         <script type="text/javascript">
         <!-- begin hiding
-        document.cookie = "Customer=\"WILE_E_COYOTE\"; Path=/acme; Version=1";
+        document.cookie = atob("{cookie_encoded}");
         // end hiding -->
         </script>
         """)
-        self.assertEqual(C.js_output(['path']), r"""
+        cookie_encoded = base64.b64encode(b'Customer="WILE_E_COYOTE"; Path=/acme').decode('ascii')
+        self.assertEqual(C.js_output(['path']), fr"""
         <script type="text/javascript">
         <!-- begin hiding
-        document.cookie = "Customer=\"WILE_E_COYOTE\"; Path=/acme";
+        document.cookie = atob("{cookie_encoded}");
         // end hiding -->
         </script>
         """)
@@ -290,17 +292,19 @@ def test_quoted_meta(self):
 
         self.assertEqual(C.output(['path']),
                          'Set-Cookie: Customer="WILE_E_COYOTE"; Path=/acme')
-        self.assertEqual(C.js_output(), r"""
+        expected_encoded_cookie = base64.b64encode(b'Customer=\"WILE_E_COYOTE\"; Path=/acme; Version=1').decode('ascii')
+        self.assertEqual(C.js_output(), fr"""
         <script type="text/javascript">
         <!-- begin hiding
-        document.cookie = "Customer=\"WILE_E_COYOTE\"; Path=/acme; Version=1";
+        document.cookie = atob("{expected_encoded_cookie}");
         // end hiding -->
         </script>
         """)
-        self.assertEqual(C.js_output(['path']), r"""
+        expected_encoded_cookie = base64.b64encode(b'Customer=\"WILE_E_COYOTE\"; Path=/acme').decode('ascii')
+        self.assertEqual(C.js_output(['path']), fr"""
         <script type="text/javascript">
         <!-- begin hiding
-        document.cookie = "Customer=\"WILE_E_COYOTE\"; Path=/acme";
+        document.cookie = atob("{expected_encoded_cookie}");
         // end hiding -->
         </script>
         """)
@@ -391,13 +395,16 @@ def test_setter(self):
             self.assertEqual(
                 M.output(),
                 "Set-Cookie: %s=%s; Path=/foo" % (i, "%s_coded_val" % i))
+            expected_encoded_cookie = base64.b64encode(
+                ("%s=%s; Path=/foo" % (i, "%s_coded_val" % i)).encode("ascii")
+            ).decode('ascii')
             expected_js_output = """
         <script type="text/javascript">
         <!-- begin hiding
-        document.cookie = "%s=%s; Path=/foo";
+        document.cookie = atob("%s");
         // end hiding -->
         </script>
-        """ % (i, "%s_coded_val" % i)
+        """ % (expected_encoded_cookie,)
             self.assertEqual(M.js_output(), expected_js_output)
         for i in ["foo bar", "foo@bar"]:
             # Try some illegal characters
diff --git a/Misc/NEWS.d/next/Security/2026-04-21-13-46-30.gh-issue-90309.srvj9q.rst b/Misc/NEWS.d/next/Security/2026-04-21-13-46-30.gh-issue-90309.srvj9q.rst
new file mode 100644
index 00000000000000..3838ac0866abf1
--- /dev/null
+++ b/Misc/NEWS.d/next/Security/2026-04-21-13-46-30.gh-issue-90309.srvj9q.rst
@@ -0,0 +1,2 @@
+Base64-encode values when embedding cookies to JavaScript to avoid injection
+and escaping.